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AlleleAnalyzer supporting data

Citation

Keough, Kathleen et al. (2019), AlleleAnalyzer supporting data, v3, UC San Francisco Dash, Dataset, https://doi.org/10.7272/Q63F4MSR

Abstract

The CRISPR/Cas system is a highly specific genome editing tool capable of distinguishing alleles differing by even a single base pair. Due to genetic variation, each patient or cell-line carries a unique set of editing sites that are not revealed by CRISPR guide RNA (sgRNA) design software that uses one reference genome. AlleleAnalyzer is open-source software that incorporates single nucleotide variants and short insertions and deletions to design sgRNAs for precisely editing one or multiple haplotypes of a sequenced genome with eleven Cas proteins. AlleleAnalyzer also leverages patterns of shared genetic variation to optimize sgRNA design for different human populations.

Methods

Please see readme and manuscript. 

Usage Notes

Please see readme and manuscript.